Journal: STAR Protocols

Article Title: Protocol for quantifying drug sensitivity in 3D patient-derived ovarian cancer models

doi: 10.1016/j.xpro.2024.103274

Figure Lengend Snippet:

Article Snippet: CD326 (EpCAM) antibody, anti-human, PE, REAfinity (flow cytometry 1:200) , Miltenyi Biotec , Cat# 130-110-999; RRID: AB_2657495.

Techniques: Immunofluorescence, Flow Cytometry, Control, Recombinant, Red Blood Cell Lysis, Saline, Software, Cell Culture, Plasmid Preparation, Microscopy

Journal: PLoS Pathogens

Article Title: Receptor-Targeted Nipah Virus Glycoproteins Improve Cell-Type Selective Gene Delivery and Reveal a Preference for Membrane-Proximal Cell Attachment

doi: 10.1371/journal.ppat.1005641

Figure Lengend Snippet: ( A ) Cell entry is EpCAM dependent but independent of ephrin-B2. Representative flow cytometry plots out of three independent experiments of CHO-K1, CHO-EpCAM, CHO-ephrin-B2 cells, and of a mixed culture composed of CHO-EpCAM and CHO-ephrin-B2 (1:1 ratio) monitored 72 h after transduction with NiVmut EpCAM -LV, NiVwt-LV or VSV-LV (MOI of 1). EpCAM expression was detected by an APC-coupled human EpCAM specific antibody. ( B ) To ascertain stability of transduction with the EpCAM-targeted vector, CHO-EpCAM cells were cultivated for further 30 days after transduction with the indicated MOIs. The percentage of GFP-positive cells was determined by flow cytometry at the indicated time points. One representative out of three independent experiments is shown.

Article Snippet: Human EpCAM was detected by an Allophycocyanin (APC) labeled mouse anti-EpCAM antibody (clone HEA-125, Miltenyi Biotec, Bergisch Gladbach, Germany, dilution 1:100).

Techniques: Flow Cytometry, Transduction, Expressing, Plasmid Preparation

Journal: Cell

Article Title: Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease

doi: 10.1016/j.cell.2018.08.067

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Article Snippet: Anti-Human CD326/EpCAM (9C4)-141Pr , Fluidigm , Cat#3141006B; RRID: AB_2687653.

Techniques: Recombinant, Coagulation, Antibody Labeling, Membrane, Plasmid Preparation, Polymer, Blocking Assay, Staining, Imaging, cDNA Synthesis, RNAscope, DNA Library Preparation, Software, Hybridization

Journal: Frontiers in Immunology

Article Title: Renshen-Baidu-San restores epithelial–immune crosstalk and drives type 2 immune repair in ulcerative colitis: an integrated multi-omics study

doi: 10.3389/fimmu.2026.1777808

Figure Lengend Snippet: BDS treatment increases tuft cell numbers and promotes type 2 immune cytokine release. (A) Representative images of immunofluorescence for GATA3 (scale bar = 50 μm). (B) Representative images of immunofluorescence for DCLK1 (scale bar = 50 μm). (C) Relative fluorescence intensity of GATA3. (D) Relative fluorescence intensity of DCLK1. (E) ELISA measurement of IL-4 concentration in colon organoids. (F) ELISA measurement of IL-13 concentration in colon organoids. (G) ELISA measurement of IL-25 concentration in colon organoids. (H) Flow cytometry analysis of tuft cell proportions in colon organoids. (I) The percentage of CD170 + CD326 + Tuft cells in colon organoids. Data are presented as the mean ± SD, n = 3. Significance levels are indicated as * p < 0.05, ** p < 0.01.

Article Snippet: HPLC grade acetonitrile, formic acid and methanol were purchased from Thermo Fisher Scientific Co., Ltd (Waltham, MA, USA); Ultrapure water was purified using UNIQUE laboratory multifunctional ultra-pure water system (Fujian, China); DSS (mw: 36–50 kDa) was purchased from MeilunBio Biological Technology Co., Ltd (Dalian, China); Collagenase I, II, and neutral protease were obtained from Sangon Biotech (Shanghai, China); The cell viability dyes Calcein AM (live), DRAQ7TM (dead) and RevertAid First Strand cDNA Synthesis Kit were purchased from Thermo Fisher Scientific Co., Ltd (Waltham, MA, USA); QubitTM dsDNA HS Assay Kit was obtained from Beckman Coulter Co., Ltd (Brea, CA, USA); Enhanced Cartridge Reagent and BD RhapsodyTM WTA Amplification Kit were obtained from BD Biosciences Co., Ltd (San Jose, CA, USA); KAPA Library Quant Kit was obtained from Illumin, Inc. (San Diego, CA, USA); Matrigel ® Matrix for Organoid Culture was obtained from Corning Inc. (NY, USA); Intestinal tissue digestion solution and organoid growth medium was obtained from Aimingmed Technologies Co., Ltd. (Hangzhou, China); Trizol-bead-based RNA extraction kit was obtained from Genstone Biotech Co., Ltd. (Beijing, China); RevertAid First Strand cDNA Synthesis Kit was obtained from Thermo Fisher Scientific Co., Ltd (Waltham, MA, USA); TB Green ® Premix Ex Taq was obtained from TakaraBio Inc. (Beijing, China); Anti-DCAMKL1, anti-Ki67 antibodies and Goat Anti-Rabbit IgG H&L (Alexa Fluor ® ) were provided by Abcam (Cambridge, MA, USA); Anti-GATA3 antibody was provided by Proteintech Group (Wuhan, China); APC Anti-Mouse CD170 and FITC Anti-Mouse CD326 antibodies were obtained from Elabscience (Wuhan, China); Elisa kit of IL-4, IL-13 and IL-25 were purchased from Jianglai Biotechnology Co., Ltd (Shanghai, China).

Techniques: Immunofluorescence, Fluorescence, Enzyme-linked Immunosorbent Assay, Concentration Assay, Flow Cytometry